UF Researchers Help Develop Highly Accurate, 30-second Coronavirus Test

The motherboard of a COVID-19 rapid testing device that UF Health researchers helped develop is seen here. The device can return a coronavirus test result as accurately and sensitively as the gold standard of testing, a PCR test, in 30 seconds. (Photo courtesy of Houndstoothe Analytics.)

By Bill Levesque

It is crucial to get a test result for a pathogen quickly, lest someone continue in their daily lives infecting others. Delays in testing have undoubtedly exacerbated the COVID-19 pandemic.

Unfortunately, the most accurate COVID-19 test often takes 24 hours or longer to return results from a lab.

At-home test kits offer results in minutes but are far less accurate.

However, researchers at the University of Florida (UF) have helped to develop a COVID-19 testing device that can detect coronavirus infection in as little as 30 seconds. The testing device is just as sensitive and accurate as a PCR or polymerase chain reaction test, the gold standard of testing. UF researchers are now working with scientists at National Yang Ming Chiao Tung University in Taiwan.

Researchers say the device could transform public health officials’ ability to quickly detect and respond to the coronavirus or the next pandemic.

UF has entered into a licensing agreement with a New Jersey company, Houndstoothe Analytics. They hope to ultimately manufacture and sell the device, not just to medical professionals but also to consumers.

According to a recent peer-reviewed study published by UF researchers, the device boasts a 90 percent accuracy rating and comparable sensitivity to that of a PCR test.

Fan Ren, Ph.D. and Josephine Esquivel-Upshaw, D.M.D. 

“There is nothing available like it,” said Josephine Esquivel-Upshaw, D.M.D., a professor in the UF College of Dentistry’s Department of Restorative Dental Sciences and a member of the research team that developed the device. “It’s true point of care. It’s access to care. We think it will revolutionize diagnostics.”

The device is not yet approved by the U.S. Food and Drug Administration. Researchers say they must first ensure that test results are not abstracted by cross-contamination. This analysis is ongoing as researchers work to identify other pathogens that might be found in the mouth and saliva that could precipitate contagion, such as other coronaviruses, staph infections, the flu, pneumonia and 20 others.

The hand-held apparatus is powered by a 9-volt battery and uses an inexpensive test strip, similar to those used in blood glucose meters. Coronavirus antibodies are attached to a gold-plated film at the tip of the device. The test strip is placed on the tongue to collect a tiny sample of saliva. The test strip is then inserted into a reader connected to a circuit board that houses the “brains” of the device.

If someone is infected, the coronavirus in the saliva binds with the antibodies and begins to dart around as they are prodded by two electrical pulses produced by a unique transistor. A higher concentration of coronavirus changes the electrical conductance of the sample which alters the voltage of the electrical pulses.

The voltage signal is amplified a million times and converted to a numerical value, which is essentially the sample’s electrochemical fingerprint. That value indicates a positive or negative result. A low value indicates a higher proportion of the virus detected within the sample. Researchers say the device’s ability to quantify viral and antibody load makes it especially useful for clinical purposes.

Esquivel-Upshaw confirms that the product can be constructed for less than $50. In contrast, PCR test equipment can cost thousands.

 The research team also is studying its ability to detect specific proteins that could be used to diagnose other illnesses, including cancer, a heart attack and immune health. 

Fan Ren, Ph.D., a distinguished professor in the Herbert Wertheim College of Engineering’s Department of Chemical Engineering and his team have been developing semiconductor-based sensor devices for nonmedical purposes long before COVID-19.

Ren notes that he finds inspiration for his work in the recent death of his wife which was unrelated to COVID-19. [SL1] He connects his grief to the mourning of the rest of the world amidst the COVID-19 pandemic.

“Almost a million people have died of COVID” in the United States, Ren said. “Those are so many tragedies. Old people. Young people. You name it. I said, ‘No, that’s it.’ That is too much.”

He told of several institutions working on devices that use a field effect transistor (FET) like that found in the COVID-19 testing device his team is developing. Even so, those devices are made for one-time use. The sample is applied directly to the FET, so the transistor is no longer usable and must be discarded.

Ren states that the expendable nature of those devices makes them expensive and impractical for mass testing.

The UF device is unique in its structure due to the separation of the transistor from the sample, like blood glucose meters that use test strips to collect a drop of blood after a lancet pierces a finger. This innovation sets the device apart from the rest in both affordability and ease of use.

Ren proposes that the device could be a crucial advancement in testing for venues with large crowds, such as concerts, sporting events or classrooms. Researchers say the unit would also potentially provide access to inexpensive and accurate testing in rural areas and developing nations.

Researchers also discussed the limitless opportunities for personal usage of the device such as parties, events, and other gatherings.

“Yes or no. You’re infected or not infected. You get the answer right away,” said Ren.


Reprinted from Today’s FDA, 2022 May/June issue. Visit floridadental.org/publications to view Today’s FDA archives.

How Coalition’s Incident Response Helps Reduce Risks During a Cyber Attack

By Leeann Nicolo with Coalition Inc.

Traditional cyber insurance protects businesses from the impact of a cyber breach after it occurs. However, what if cyber coverage could actually help reduce the risk of an attack before it happens? 

As cyber incidents increase across the globe — cyber claims severity rose 56% for small businesses last year — reducing the risk of an attack proactively is critical to reducing overall business risk. 

Enter: Coalition’s Incident Response (CIR)

Driven by a team of technical experts, incident responders, forensic specialists, and security engineers, Coalition provides security across the lifecycle of a business, elevating the ability to respond to bad actors, including a security support center to help prevent attacks before they happen — and respond more effectively if they do.

From ransomware to reputational impacts, CIR solved 46% of incidents reported to Coalition last year —  without additional costs or using up policyholder deductibles. Even more consequential, CIR helped many policyholders prevent cyber incidents last year, as Coalition’s insureds experience less than one-third the frequency of claims compared to the broader cyber insurance market (based on 2020 and 2021 National Association of Insurance Commissioners [NAIC] report data).

The incident timeline: investigate, remediate, communicate

When a cyber incident does occur, policyholders should be encouraged to report the incident to their insurance provider immediately. 

Reporting an incident gets the ball rolling and alerts all necessary vendors and experts to mobilize, with the goal of reducing the overall impact of the incident. Coalition’s Claims team responds immediately to help determine what services to activate, from forensics specialists to a breach coach, and/or a PR firm to manage crisis communications. 

The Coalition team will walk an insured through an investigation and remediation of the incident, while also working on all points of critical communication simultaneously. Here’s the Coalition Incident Response timeline as seen through an investigate-remediate-communicate lens: 

  1. Investigate what happened, to determine the tactics and techniques used by the threat actor during the incident. Building off of the insured’s Active Risk Assessment — a scan of how the insured’s network is seen on the dark web, so all vulnerabilities are visible — CIR collects and analyzes forensic artifacts and system logs to dive into what vulnerabilities may have enabled the incident and how the business can react to protect itself. This includes determining if the business has available backups and utilizing tools to have oversight and block the threat actors from gaining more access. Were any previous vulnerabilities noted and not patched? Were all the potential protections implemented in good time? While Coalition’s in-house team leads the investigation of the cyber incident, based on its complexity, third-party specialists could be also called in to supplement Coalition’s expertise. 
  1. Remediate for both the short- and long term. While remediation steps are informed by the investigation, this step happens alongside the investigation. The goal here is to act quickly in order to minimize the damage. CIR recommendations will be based on what is known and learned about the business, as well as on protection implementations recommended to them during earlier stages of the Active Insurance life cycle.  For example, if the business has viable backups in place for all of their critical data, CIR can guide the business through a process that avoids interacting with the bad actor and gets them back online more quickly. As a general rule of thumb, offline backups offer the greatest chance of survival during a cyber incident, because they’re unreachable to the threat actor. Online backups are often also seized or locked up by the threat actors along with your network. The CIR team guides insureds through the entire process of restoring from backup, from negotiation, to testing encrypted data, and finally (if necessary) paying the threat actors for a decryption key to regain control of the network. Remediation is also a time during which CIR will make network recommendations for the future. These may include: 
    • Multi-factor authentication (MFA), the electronic authentication of two or more pieces of evidence in order to be granted access to a website or application 
    • Endpoint detection response (EDR), software installed on all servers and endpoints, designed to stop ransomware and identify unusual behavior in an application
    • Network segmentation, a strategy that provides limited network access to employees, based on job qualifications, tasks or seniority. This helps businesses reduce network access. 
  1. Communicate the technical details. CIR will act as the business’s technical expert during the incident’s communication process. This includes communicating with the bad actors if necessary, but also providing the appropriate technical detail to include in internal and external communications about the incident.  For example, depending on the regulatory laws in effect in the states in which the business operates, CIR will provide guidance on what needs to be communicated to anyone with breached data. CIR can also work with the policyholder to provide an accurate understanding of how much the claim will cost based on the remediation necessary and forensic investigation that took place.

Very often during and after a cyber incident, it’s important to engage a special public relations firm that deals with cyber incidents and their crisis communications needs. Coalition’s cyber coverage includes this service, and such a team is engaged if necessary by the CIR team as well. 

Coalition Incident Response is active

Coalition’s Active Insurance goes beyond a cyber insurance standard, playing an integrated role in your business’ cybersecurity across its lifecycle. It’s like combining the safety features of a vehicle — reverse backup sensors or automatic braking — with the post-accident coverage. 

Coalition’s Active Insurance approach consists of Active Risk Assessment, which takes place before the policy is written, Active Protection during the policyholder period, and Active Response, a post-breach response. All three phases of this lifecycle provide a continuous feedback loop of the business’ current risk level and vulnerabilities.

To report a cyber incident, Coalition policyholders can reach CIR here.

To learn more about Active Insurance, download the Coalition Active Insurance eBook.

Reprinted from Coalition Inc. Visit coalitioninc.com/blog to view the rest of the content on Coalition’s blog.

Chew on This: Dental Supply Best Practices with TDSC.com

Do you ever wonder what drives the cost of dental supplies? Want to know how to best keep supply costs low or if we should expect another shortage of personal protective equipment? Were you aware that all Florida Dental Association (FDA) members are part of a group purchasing organization and eligible for exclusive discounts?

FDA Executive Director Drew Eason gets answers to all these questions and more during his “Chew on This” interview with representatives of TDSC.com, powered by Henry Schein. Watch the interview!

Learn more by visiting TDSC.com and, if you’re attending FDC 2022, stop by the TDSC booth (Booth 430) to meet the TDSC team and play a game to win. 

Today’s FDA is Available to Read Online NOW!

The May/June 2022 issue of Today’s FDA is available online for FDA members NOW! Check it out for great articles about the Florida Dental Convention (FDC) and what to know before you go, artificial intelligence (AI) in today’s dentistry and, of course, special edition FDC speaker previews. So, what are you waiting for? Head to floridadental.org/publications or just click the cover image below to read the issue.